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KMID : 0361319840160010051
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Korean Journal of Medical Technologists
1984 Volume.16 No. 1 p.51 ~ p.56
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Evaluation of C₃, C₄ in Serum by Turbidimetric Quantitation Using a Centrifugal Analyzer
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Abstract
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The complement acts as a part of immune system to protect the individual from microbial infection by mediating a variety biologic reaction; opsonization, chemotaxis of leukocytes, increased vascular permeability, and cytolysis of target organisms.
The complement system is consisted about 18 proteins, among them, The C, and C, are the highest concentrates in serum. These proteins are said to be in "classic pathway" or "alternative pathway" names.
Activation of the complement proteins result in loss of their precursors, native activities and, or their accelerated clearence from plasma. Progressing hypercatabolism -without compensation by increased synthesis of complements, the determination of depressed levels of them may provide the evidence of the complement system activation.
For the evaluation of the patient with autoimmune disease such as autoimmune hemolytic anemia, SLE, rheumatoid arthritis and poststreptococcal glomerulonephritis, the determination of C., and C, concentration are both of the most informative method.
Low concentration of C. indicated that classic pathway activation has occurred, since C, is extremely sensitive to C,. Depressed level of C, suggests that rather intense activation of ether path-way. Decreased C3 associated with normal C, levels indicate exclusive activation of alternative pathway.
During the past decale, a number of invastigators have developed the techniques for measurement of complement. Since 1929, Michael Heidleberger has developed immunoprecipitation analysis of complement. And also Boyden et al (1947) demonstrated turbidimetric absorbance using the basis of antigen quantitation. The radial immunodiffusion (RID) technique has proved to be one of the most useful methods in clinical laboratories during the past´few years, but it require rather long processes from 18 to 48 hrs in order to complete precipitation ring formation.
So we attempt to introduce and establish more rapid completion of complement quantitation based on turbidimetric technique using a centrifugal analyzer.
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